Douglas Costa and Danilo have been ruled out of Brazil's last World Cup group game against Serbia due to injury.
The duo will not travel with the rest of the squad for Wednesday's closing Group E clash in Moscow.
Danilo started Brazil's 1-1 opening draw against Switzerland but missed their 2-0 win over Costa Rica due to a hip problem, with Fagner deputising.
Costa was influential off the bench as late goals from Philippe Coutinho and Neymar downed Costa Rica on Friday.
But the Juventus winger will not be available for the Serbia match as he has a hamstring injury, team doctor Rodrigo Lasmar confirmed.
"At the end of yesterday's game, Douglas Costa complained of pain in his right hamstring," Lasmar told reporters. "Just remember he arrived injured, but in the left leg. That is different from this complaint.
"He was diagnosed with a minor muscle injury so he will not travel with us. He will stay in Sochi, where we have a structure suitable for him to recover as soon as possible.
"We'll have a physiotherapist here. He will continue the recovery process here, along with Danilo, who will not travel and will have physiotherapy aimed at recovery.
"We are optimistic about his participation in the competition, depending on our performance at the World Cup. We are sparing no effort so can he still help us.
"The case that is most worrying is Douglas Costa's, but Danilo's is not much different, except that his is a less important, secondary muscle, so there's a little better expectation with respect to him."
Lasmar, though, is not willing to speculate on when Costa may be ready to return to action, with Brazil needing a point against Serbia to guarantee a last-16 spot.
"We are not going to need a date when he will be ready," Costa said.
"It is clear he is not in condition for this game and his recovery will tell us. We are imagining within a favourable time in the competition."
|´No question´ Fitzgerald faces Bears, says Cardinals´ Wilks|
|Rodgers says roughing the passer penalties are ´going the wrong direction´|
|Ravens fined $200,000 for violating communication policy|
|LeBron James set to star in ´Space Jam´ sequel|
|Ronaldo red card ´absurd´, says Pjanic|
|Kovac hails ´special´ Sanches after Bayern goal|
|Marcelino: Tearful Ronaldo said he did nothing wrong|
|Fernandinho takes blame for Man City´s Lyon loss|
|Lovren denies any wrongdoing after perjury charge|
|Plzen boss Vrba slams late mistakes|
|I almost scored as many as Messi - Tagliafico the unlikely hero for Ajax|
|Ronaldo deserves Ballon d´Or not Modric, says Casemiro|
|United fan Rafael - I´ve never lost at City|
|Davis explains abrupt mid-game NFL retirement: ´Football is not for me´|
|Lopes pays tribute to under-fire Lyon coach Genesio|
|Toulon´s Bastareaud handed five-week ban|
|Steelers WR Brown disciplined for missing meeting|
|Arteta refuses to make excuses for Manchester City´s Lyon loss|
|Allegri calls for VAR after Ronaldo´s contentious red card|
|Championship Review: Boro go second, Birmingham still winless|
|Lopetegui: Mariano took his chance|
|Juventus proved their quality after Ronaldo red card - Bonucci|
|Pogba ´class´ impresses Mourinho|
|Man City not Champions League favourites, insists Bernardo Silva|
|Isco calls for Modric to win Ballon d´Or after Champions League win|
|Wawrinka into last eight in St Petersburg|
|Goal hero Pogba is one of the best in the world - Shaw|
|Sane SOS can´t salvage sorry City display|
|Hoffenheim will learn lessons from Shakhtar draw, insists Nagelsmann|
|Valencia 0 Juventus 2: Pjanic hits the spot after Ronaldo sees red|
|Viktoria Plzen 2 CSKA Moscow 2: Krmencik heroics undone by late show|
|Benfica 0 Bayern Munich 2: Sanches returns to haunt his former club|
|Young Boys 0 Manchester United 3: Pogba double inspires Red Devils|
|Manchester City 1 Lyon 2: Cornet and Fekir stun Champions League favourites at home|
|Real Madrid 3 Roma 0: Isco stunner sets up winning start for Champions League holders|
|Ronaldo sent off on Champions League debut for Juve and may miss United reunion|
|Shakhtar Donetsk 2 Hoffenheim 2: Maycon denies Nagelsmann´s side|
|Ajax 3 AEK Athens 0: Tagliafico at the double in opening win|
|Dalot makes debut, Martial starts for Manchester United|
|India avenge ICC Champions Trophy loss with big win over Pakistan|
|Ronaldo makes Champions League debut for Juve|
|Lopetegui picks Navas over Courtois for Champions League clash with Roma|
|Willian very happy under Sarri after Conte row|
|Sarri deals blow to Chelsea kids´ Europa League hopes|
|Mendy out for Manchester City as Aguero makes bench|
|Cech accepts performances key to securing Arsenal stay|
|Monchi rules out Spain return amid Barcelona rumours|
|Durham skittled for 61 and 66 on the same day|
|Kane isn´t a machine – Lamela defends misfiring Spurs star|
|Hazard left out of Chelsea squad for PAOK trip|
|Tiger feels close to ending drought after achieving Tour Championship goal|
|Leno in line for Arsenal chance in Europa League|
|Gazidis leaves Arsenal in good shape, insists Emery|
|Eagles bring back WR Matthews, place Wallace on IR|
|England include Stokes, Hales and uncapped seamer Stone in ODI squad|
|Suwon Bluewings 0 Jeonbuk Motors 3 (aet, 4-2 on penalties): Quarter-final comeback ends in spot-kick|
|Australia captain Paine ready to slog it out with Pakistan|
|Nadal pulls out of China Open & Shanghai Masters with knee injury|
|Ostapenko begins Korea Open defence with victory|
|Joshua v Povetkin: How Olympic super heavyweight champions have fared|
|Liverpool ´made a point´ with PSG victory - Gomez|
|Osaka wows Tokyo crowd on WTA return|
|Excited Povetkin arrives in England for Joshua showdown|
|Kovac patience with Sanches set to reap rewards|
|Kovac: Madrid strong without Ronaldo, Messi is the best|
|ATP suspends umpire Lahyani over Kyrgios ´pep talk´ at US Open|
|North and South Korea seek to co-host 2032 Olympics|
|Ampadu signs long-term Chelsea contract|
|Don´t blame Neymar, PSG players all guilty - Thiago Silva|
|Real Madrid seek to plug Ronaldo scoring gap – Champions League in Opta numbers|