Douglas Costa and Danilo have been ruled out of Brazil's last World Cup group game against Serbia due to injury.
The duo will not travel with the rest of the squad for Wednesday's closing Group E clash in Moscow.
Danilo started Brazil's 1-1 opening draw against Switzerland but missed their 2-0 win over Costa Rica due to a hip problem, with Fagner deputising.
Costa was influential off the bench as late goals from Philippe Coutinho and Neymar downed Costa Rica on Friday.
But the Juventus winger will not be available for the Serbia match as he has a hamstring injury, team doctor Rodrigo Lasmar confirmed.
"At the end of yesterday's game, Douglas Costa complained of pain in his right hamstring," Lasmar told reporters. "Just remember he arrived injured, but in the left leg. That is different from this complaint.
"He was diagnosed with a minor muscle injury so he will not travel with us. He will stay in Sochi, where we have a structure suitable for him to recover as soon as possible.
"We'll have a physiotherapist here. He will continue the recovery process here, along with Danilo, who will not travel and will have physiotherapy aimed at recovery.
"We are optimistic about his participation in the competition, depending on our performance at the World Cup. We are sparing no effort so can he still help us.
"The case that is most worrying is Douglas Costa's, but Danilo's is not much different, except that his is a less important, secondary muscle, so there's a little better expectation with respect to him."
Lasmar, though, is not willing to speculate on when Costa may be ready to return to action, with Brazil needing a point against Serbia to guarantee a last-16 spot.
"We are not going to need a date when he will be ready," Costa said.
"It is clear he is not in condition for this game and his recovery will tell us. We are imagining within a favourable time in the competition."
|Arsenal chief confirms Super League talks|
|Hooker knocks out Saucedo to retain WBO strap|
|NBA wrap: Kyrie Irving sizzles as Celtics beat Raptors in overtime|
|Jimmy Butler aims dig at Timberwolves following 76ers home debut|
|Koeman surprised by dominant Netherlands|
|Southgate rallies England ahead of Nations League decider|
|Chile 2 Costa Rica 3: Waston brace sinks La Roja|
|Tite inspired by Guardiola´s City|
|Argentina 2 Mexico 0: Funes Mori´s header helps to sink El Tri|
|Lovren ready for ´fiery´ England clash|
|Brazil captain Neymar pleased with stern Uruguay test|
|Ravens´ Flacco doubtful, Jackson could start|
|Richarlison: I want to be at the Copa America|
|Tabarez buoyed by inexperienced Uruguay|
|Howell fires 64 again to extend RSM Classic lead|
|I´m proud of them - Giggs still upbeat despite Denmark defeat|
|Mancini ´very sorry´ for Ronaldo absence|
|Neymar clinches anything-but-friendly win for Brazil over Uruguay|
|Cipriani-inspired Gloucester thrash Tigers|
|Djokovic makes it three wins from three at ATP Finals|
|Germany relegated from Nations League top tier to cap miserable 2018|
|Fulham will back Ranieri in January transfer window if required|
|Brazil 1 Uruguay 0: Neymar penalty settles tight contest|
|Netherlands 2 France 0: Wijnaldum & Depay decisive as Germany are relegated|
|Wales 1 Denmark 2: Jorgensen and Braithwaite seal Nations League promotion|
|Oxlade-Chamberlain targeting early return|
|New Zealand-born Aki will ´relish´ facing All Blacks – Marmion|
|Portugal cannot focus on absent Ronaldo - Santos|
|Ramsey reiterates he wants to stay with Jaguars despite cryptic tweet|
|We tried to hit a home run and it didn´t work out - D´Antoni explains Carmelo exit|
|West Ham´s Zabaleta backs Premier League´s VAR introduction|
|Southgate eyes winning end to England´s ´brilliant year´|
|Patient Zverev seizes opportunity to make amends at ATP Finals|
|Rodgers: Packers still believe they can make playoffs|
|Mahomes hoping to ketchup to Heinz´s touchdown target|
|Ranieri tells Fulham fans not to expect repeat of Leicester ´fairytale´|
|Brown to leave role as RFU chief executive|
|Croatia star Rakitic ruled out of England clash|
|Capital gains for Zverev as he secures last-four spot in London|
|Sancho rise continues with Player of the Month award|
|Jones-Buchanan, Ablett and Briscoe sign new Rhinos deals|
|Root leads from the front as aggressive England assume control|
|Croatia´s Lovren antagonises Ramos and ´rude´ Spain|
|Rakitic has ´enormous respect´ for England ahead of Nations League decider|
|Ireland make flanker switch with Leavy out of All Blacks clash|
|Pakistan rip through Black Caps in Abu Dhabi|
|WATCH: Barcelona mark 15-year anniversary of Messi´s first match|
|Sterling, that you? - Walker uses falling fan to poke fun at England team-mate|
|Fleetwood applies pressure on Molinari, Wallace leads in Dubai|
|Don´t talk to me about VAR – Rakitic not over World Cup final loss|
|Van Dijk leadership essential for Liverpool – Wijnaldum|
|Dalic: Croatia´s bench was ready to stop Spain!|
|Sublime Root century gives England imposing lead|
|Vinicius appreciates Solari influence at Real Madrid|
|Havertz would suit Bayern Munich – Kimmich|
|Wilder: Roach recruitment a sign of Fury nerves|
|Ranieri targets defensive improvements at failing Fulham|
|PSG close investigation into player recruitment practices after finding no discrimination|
|Mayweather: Nasukawa exhibition back on|
|Witsel: Joining Borussia Dortmund my best career decision|
|Ballon d´Or win would not change Modric´s ´best year´|
|Ireland v New Zealand: Opta stats behind the meeting of the world´s top two|
|The Breakdown: A statistical look at Saturday´s rugby internationals|
|Batshuayi dismisses reports of Valencia departure|
|Brazil must make Copa America final – Tite|
|Rockets rout Warriors as Green goes scoreless|
|Seahawks rally past Packers|
|Lakers G Rondo to miss 4-5 weeks|
|Durant is done with questions about Green|
|Smith, Warner set to return to IPL in 2019|